Plant and Soil Science

In this work, samples of sunflower seeds and samples of sunflower seeds, after artificial contamination with solutions of polycyclic aromatic hydrocarbons group xenobiotics analytical standards (PAHs: benzo(a)anthracene, chrysene, benzo(a)pyrene, benzo(b)fluoranthenum) were investigated. The optimum conditions for homogenization of samples and conditions for xenobiotics extraction by means of maceration method are determined, optimal conditions are proposed for carrying out the procedure for preparing samples of sunflower seeds for the study of surfactant content. The investigated method of preparation of samples consists in obtaining an oil extract from a chloroform extract which was obtained by maceration, intensified by constant stirring at a rate of 200 revolutions per minute during 3 hours at a chloroform ratio:raw material equal to 1:10. Pieces are shredded to size of ≤ 2,0 mm of sunflower seeds used for extraction. The quantitative and qualitative composition of the oil extracts obtained from the lipophilic xenobiotics of the polycyclic aromatic hydrocarbons group was investigated by the method of high-performance liquid chromatography with ultraviolet detector (HPLC/FLD) developed for surfactant laboratory control in vegetable oils at the NULES of Ukraine structural depatrment. In the oil extracts of samples of sunflower seeds, which were not artificially enriched with surfactants, a list of normalized xenobiotics of the group of polycyclic aromatic hydrocarbons was found. 

xenobiotics, polycyclic aromatic hydrocarbons, sunflower seeds, extraction, high performance liquid chromatography